Biological activity of flavonoids from Sonchus brachyotus

The aim of this study was to isolate and identify secondary metabolites from Sonchus brachyotus evaluate their antioxidant anti-inflammatory activities. In study, we isolated three flavonoids a 70% EtOH extract by Medium Pressure Liquid Chromatography (MPLC) prep-High-Performance (HPLC). To the biological activities (an-tioxidant anti-inflammatory) these flavonoids, in vitro inhibitory against lipopolysaccharide (LPS)-induced reactive oxygen species (ROS) generation, nitric oxide (NO) production, prostaglandin E2 (PGE2) secretion were determined. We successfully identified namely luteolin (1), luteolin-7-O-β-D-glucoside (2), luteolin-7-O-β-D-glucuronide (3) spectral analyses. Luteolin (1) at 20 μg/mL inhibited ROS NO PGE2 48.6%, 61.28% 12.10%, respectively, (2) generation 67.03% 20.82%, respectively. showed similar activities; however, observed be stronger antioxidant. Besides activities, S. containing lu-teolin-7-O-β-D-glucoside is considered possess diverse results indicate that an edible medicinal plant, which believed significant resource functional foods.